The Royal Canadian Mounted Police in Manitoba confirmed the latest death from last Thursday’s crash.
The group of 25 people were on a day-trip to a casino from rural Dauphin, Manitoba, when the crash happened on the Trans-Canada Highway.
Health officials say nine patients remain in hospital. Four are in a critical condition.
Police have said dashcam footage shows the bus was crossing the Trans-Canada Highway when it went into the path of the truck.
They said officers have not yet interviewed the bus driver as he is receiving care in hospital.
The police investigation continues.